對(dui)于(yu)很多(duo)科研汪來(lai)說，細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)是脆弱又重(zhong)要的(de)(de)寶(bao)貝(bei)之一(yi)(yi)(yi)，承擔(dan)著重(zhong)要的(de)(de)實驗數據希望(wang)。尤其是在(zai)凍(dong)(dong)存(cun)(cun)(cun)(cun)復(fu)蘇的(de)(de)時候，經常會因為一(yi)(yi)(yi)些小細(xi)(xi)(xi)節(jie)導致問題。比如：沒有(you)控制好(hao)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)的(de)(de)生長密(mi)度細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)的(de)(de)密(mi)度對(dui)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)的(de)(de)生存(cun)(cun)(cun)(cun)質量有(you)著重(zhong)要的(de)(de)影響，畢(bi)竟它(ta)們是一(yi)(yi)(yi)群(qun)又不(bu)能擠著了也不(bu)能孤(gu)獨的(de)(de)嬌貴寶(bao)寶(bao)。密(mi)度過高會讓細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)沒有(you)足夠的(de)(de)生存(cun)(cun)(cun)(cun)空間(jian)，密(mi)度過低會讓細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)無法互相連接健(jian)康生長。建議大(da)家在(zai)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)接種前，一(yi)(yi)(yi)定(ding)要調整(zheng)好(hao)適合細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)生長的(de)(de)濃度，提高細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)的(de)(de)存(cun)(cun)(cun)(cun)活率。溫(wen)度控制不(bu)達(da)標慢(man)凍(dong)(dong)快融是細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)存(cun)(cun)(cun)(cun)復(fu)蘇的(de)(de)*關鍵詞之一(yi)(yi)(yi)存(cun)(cun)(cun)(cun)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)時需(xu)現配(pei)凍(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)(如購買市面上通用的(de)(de)含血清(qing)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)。杭州長沙無血清(qing)細(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)

無(wu)(wu)血(xue)(xue)清(qing)(qing)(qing)細(xi)(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)存(cun)(cun)(cun)液，含多種保護(hu)劑成分(fen)。一(yi)些保護(hu)劑，易于穿透細(xi)(xi)(xi)(xi)胞(bao)(bao)，避免細(xi)(xi)(xi)(xi)胞(bao)(bao)內(nei)部水(shui)分(fen)子(zi)形(xing)成冰晶損(sun)傷(shang)細(xi)(xi)(xi)(xi)胞(bao)(bao)，同時另一(yi)些保護(hu)劑不能(neng)穿透細(xi)(xi)(xi)(xi)胞(bao)(bao)，但可(ke)以在(zai)冰晶形(xing)成之前(qian)，優先結合細(xi)(xi)(xi)(xi)胞(bao)(bao)外(wai)(wai)的水(shui)分(fen)子(zi)，降低細(xi)(xi)(xi)(xi)胞(bao)(bao)外(wai)(wai)溶液的電解質濃(nong)度(du)，減少陽離子(zi)進入細(xi)(xi)(xi)(xi)胞(bao)(bao)的數量(liang)(liang)，為多種保護(hu)劑組合，在(zai)細(xi)(xi)(xi)(xi)胞(bao)(bao)內(nei)外(wai)(wai)進行雙重保護(hu)，較(jiao)大提(ti)高了細(xi)(xi)(xi)(xi)胞(bao)(bao)復(fu)蘇存(cun)(cun)(cun)活率(lv)。無(wu)(wu)血(xue)(xue)清(qing)(qing)(qing)細(xi)(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)存(cun)(cun)(cun)液不含牛血(xue)(xue)清(qing)(qing)(qing)，無(wu)(wu)動(dong)物源(yuan)組份。2-8℃即可(ke)穩定保存(cun)(cun)(cun)，無(wu)(wu)需冷凍(dong)(dong)，即取即用(yong)。凍(dong)(dong)存(cun)(cun)(cun)細(xi)(xi)(xi)(xi)胞(bao)(bao)，無(wu)(wu)需程序(xu)降溫。凍(dong)(dong)存(cun)(cun)(cun)細(xi)(xi)(xi)(xi)胞(bao)(bao)復(fu)蘇率(lv)在(zai)90%以上。重慶正規無(wu)(wu)血(xue)(xue)清(qing)(qing)(qing)細(xi)(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)存(cun)(cun)(cun)液廠家批發(fa)價(jia)無(wu)(wu)血(xue)(xue)清(qing)(qing)(qing)細(xi)(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)存(cun)(cun)(cun)液使(shi)用(yong)方(fang)法(fa)：加入適(shi)量(liang)(liang)的無(wu)(wu)血(xue)(xue)清(qing)(qing)(qing)型細(xi)(xi)(xi)(xi)胞(bao)(bao)凍(dong)(dong)存(cun)(cun)(cun)液于離心管中(zhong)。

無(wu)(wu)血清細(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)存液的(de)(de)操(cao)(cao)作規范：1、培(pei)養(yang)細(xi)(xi)胞(bao)(bao)(bao)(bao)至對數(shu)生(sheng)長(chang)(chang)晚期(qi)，顯微鏡(jing)觀察其外觀、形態、有無(wu)(wu)污(wu)染等(deng)。選擇(ze)狀態良好的(de)(de)細(xi)(xi)胞(bao)(bao)(bao)(bao)進行凍(dong)存操(cao)(cao)作(注：貼壁生(sheng)長(chang)(chang)細(xi)(xi)胞(bao)(bao)(bao)(bao)，用(yong)胰蛋白酶消(xiao)化并用(yong)完(wan)全培(pei)養(yang)基終(zhong)止(zhi)消(xiao)化，進行細(xi)(xi)胞(bao)(bao)(bao)(bao)計數(shu);懸浮生(sheng)長(chang)(chang)細(xi)(xi)胞(bao)(bao)(bao)(bao)，進行細(xi)(xi)胞(bao)(bao)(bao)(bao)計數(shu))。2、500～1000g離心5min，棄上清。3、加(jia)入適量(liang)的(de)(de)細(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)存液，重懸細(xi)(xi)胞(bao)(bao)(bao)(bao)，使細(xi)(xi)胞(bao)(bao)(bao)(bao)濃度達到1～10×106/ml，置于凍(dong)存管(guan)中密閉。4、采取逐(zhu)級(ji)降溫保存：4℃放置20min，-20℃放置30min，-70℃過夜，置于液氮罐(guan)中長(chang)(chang)期(qi)存儲。注意(yi)：務必超凈工作臺(tai)內無(wu)(wu)菌(jun)操(cao)(cao)作，避免(mian)污(wu)染。雜交瘤(liu)細(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)存時(shi)應定(ding)期(qi)復蘇，以檢查細(xi)(xi)胞(bao)(bao)(bao)(bao)的(de)(de)活性(xing)和分泌抗體的(de)(de)穩定(ding)性(xing)。

無(wu)血清細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)(dong)存(cun)(cun)(cun)液(ye)通用于各種動物細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)株(zhu)（tumour細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)和常規細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)），大部(bu)分的干細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，凍(dong)(dong)存(cun)(cun)(cun)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)可(ke)(ke)在-80℃長期(qi)保(bao)(bao)存(cun)(cun)(cun)。不含(han)動物源性蛋白，不含(han)血清成分，可(ke)(ke)有(you)效減(jian)少各類細(xi)(xi)(xi)(xi)菌(jun)、病毒和支原體等污(wu)染(ran)，保(bao)(bao)證凍(dong)(dong)存(cun)(cun)(cun)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)的安全。該凍(dong)(dong)存(cun)(cun)(cun)液(ye)含(han)DMSO、葡萄糖等各種細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)營養成分，提高細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)存(cun)(cun)(cun)活(huo)率和活(huo)力，亦(yi)適(shi)合于無(wu)血清培養細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)和蛋白表達(da)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)。產(chan)品優(you)勢：1、無(wu)需(xu)(xu)程序性降(jiang)溫，可(ke)(ke)直接將細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)置于-80℃凍(dong)(dong)存(cun)(cun)(cun)，凍(dong)(dong)存(cun)(cun)(cun)液(ye)無(wu)需(xu)(xu)稀釋(shi)。2、細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)可(ke)(ke)于-80℃長期(qi)保(bao)(bao)存(cun)(cun)(cun)。3、凍(dong)(dong)存(cun)(cun)(cun)液(ye)不含(han)血清等，較大降(jiang)低細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)污(wu)染(ran)的可(ke)(ke)能性。保(bao)(bao)存(cun)(cun)(cun)方法：冰袋(dai)運(yun)輸(shu)，4℃保(bao)(bao)存(cun)(cun)(cun)，保(bao)(bao)質期(qi)一年。無(wu)血清細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)(dong)存(cun)(cun)(cun)液(ye)可(ke)(ke)用于其他類型哺(bu)乳動物細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)的儲存(cun)(cun)(cun)。

無血(xue)(xue)清(qing)(qing)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)液(ye)的(de)(de)(de)(de)使(shi)用(yong)方法(fa)及(ji)注意事項：無血(xue)(xue)清(qing)(qing)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)液(ye)：含多(duo)種(zhong)保(bao)(bao)(bao)(bao)護(hu)(hu)劑成分(fen),一些(xie)(xie)保(bao)(bao)(bao)(bao)護(hu)(hu)劑,易于穿透細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao),避免細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)內(nei)部水(shui)分(fen)子(zi)形成冰晶損傷細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao),同(tong)時(shi)另一些(xie)(xie)保(bao)(bao)(bao)(bao)護(hu)(hu)劑不能(neng)穿透細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao),但可以在冰晶形成之前,優先(xian)結合細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)外(wai)的(de)(de)(de)(de)水(shui)分(fen)子(zi),降(jiang)低細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)外(wai)溶液(ye)的(de)(de)(de)(de)電(dian)解質濃(nong)度(du)(du),減少(shao)陽離子(zi)進入(ru)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)數(shu)量,我公司無血(xue)(xue)清(qing)(qing)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)液(ye),為多(duo)種(zhong)保(bao)(bao)(bao)(bao)護(hu)(hu)劑組合,在細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)內(nei)外(wai)進行雙(shuang)重保(bao)(bao)(bao)(bao)護(hu)(hu),較大提高了細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)復(fu)蘇存(cun)(cun)(cun)活(huo)率(lv)。【產品用(yong)途】1.用(yong)于免疫(yi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)及(ji)干細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)存(cun)(cun)(cun)儲。2.細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)保(bao)(bao)(bao)(bao)藏中(zhong)(zhong)心，用(yong)于細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)株的(de)(de)(de)(de)長期保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。3.科研高校，細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)株凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)。4.醫院(yuan)樣(yang)本(ben)庫細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)樣(yang)本(ben)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。【使(shi)用(yong)方法(fa)】1、細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)前準(zhun)備(bei)（1）要求凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)的(de)(de)(de)(de)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)在對數(shu)生長期，且活(huo)率(lv)大于90%。（2）計(ji)算(suan)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)密(mi)度(du)(du)，一般要求密(mi)度(du)(du)在1-3x10cells/mL，不同(tong)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)系請參照附表。2、細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)過程（1）將要凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)的(de)(de)(de)(de)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)懸(xuan)液(ye)，進行細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)計(ji)數(shu)，計(ji)數(shu)后(hou)離心，8003min即可。度(du)(du)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)的(de)(de)(de)(de)無血(xue)(xue)清(qing)(qing)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)液(ye)緩慢加入(ru)離心后(hou)的(de)(de)(de)(de)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)沉淀中(zhong)(zhong)，根據密(mi)度(du)(du)調(diao)整細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)液(ye)用(yong)量。（3）反復(fu)吹吸混勻后(hou)，分(fen)裝于細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)管(guan)中(zhong)(zhong)，每管(guan)500ul-1000ul，（建(jian)議500ul/管(guan)）。無血(xue)(xue)清(qing)(qing)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)液(ye)使(shi)用(yong)方法(fa)：將凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)管(guan)直接轉移(yi)至-80C水(shui)箱中(zhong)(zhong)冷凍(dong)(dong)(dong)(dong)保(bao)(bao)(bao)(bao)存(cun)(cun)(cun)。廣州正規無血(xue)(xue)清(qing)(qing)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)(dong)存(cun)(cun)(cun)液(ye)廠家直銷

可以(yi)防止或減少冷(leng)凍冰晶對細(xi)胞的損(sun)傷作用。杭州長(chang)沙(sha)無血清(qing)細(xi)胞凍存液

無(wu)(wu)(wu)(wu)血(xue)清(qing)快速細胞(bao)凍(dong)存(cun)液(ye)，通(tong)用(yong)于(yu)各(ge)種動(dong)物細胞(bao)株。特別(bie)配(pei)方具有有效(xiao)提高細胞(bao)凍(dong)存(cun)活率和(he)復(fu)蘇(su)活力。不含動(dong)物來源性蛋(dan)白(bai)，能(neng)減少各(ge)類(lei)病毒(du)、霉(mei)菌和(he)支原體等的污染，確保凍(dong)存(cun)細胞(bao)安全(quan)。既適用(yong)于(yu)一般(ban)培(pei)養細胞(bao)的凍(dong)存(cun)，也適用(yong)于(yu)無(wu)(wu)(wu)(wu)血(xue)清(qing)培(pei)養細胞(bao)和(he)蛋(dan)白(bai)表達細胞(bao)的凍(dong)存(cun)。產品特色：高安全(quan)性，完全(quan)使用(yong)醫(yi)藥品等級原料進(jin)行(xing)生(sheng)產，不含動(dong)物成分，病毒(du)、霉(mei)菌和(he)支原體等污染可(ke)能(neng)性低(di)，各(ge)批產品之(zhi)間(jian)有更高的產品質量一致性。細胞(bao)存(cun)活率高，無(wu)(wu)(wu)(wu)批次差異。完全(quan)凍(dong)存(cun)液(ye)配(pei)方，可(ke)直(zhi)接使用(yong)，方便簡捷，可(ke)直(zhi)接存(cun)放于(yu)-70℃冰箱凍(dong)存(cun)，無(wu)(wu)(wu)(wu)需(xu)經(jing)過費時(shi)的程(cheng)序(xu)降溫(wen)過程(cheng)（省(sheng)時(shi)、省(sheng)力、省(sheng)錢）。杭州長(chang)沙無(wu)(wu)(wu)(wu)血(xue)清(qing)細胞(bao)凍(dong)存(cun)液(ye)

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